Protein_Domain

Part:BBa_K4638020:Design

Designed by: Hao Yu   Group: iGEM23_PekingHSC   (2023-10-11)


EX-NA-MUT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 111
    Illegal PstI site found at 944
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 111
    Illegal PstI site found at 944
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1118
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 111
    Illegal PstI site found at 944
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 111
    Illegal PstI site found at 944
    Illegal NgoMIV site found at 219
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1414


Design Notes

Influenza should be carefully engineered. If you want to use this composite part in other types of Influenza, you need to do the same way of mutation in both the protein and the packaging signal so that the virus can be successfully packaged.


Source

Extracted from the DNA sequence of WSN from NIH.

References